Bound impurities are removed from the beads by cleaning-in-place (CIP) procedures using NaOH and in most cases, a solvent. Capto Core 700 is a core-shell chromatographic support with an adsorbing core contained within an inert shell layer designed to purify larger biomolecules and bioparticles in a flow-through mode.The multimodal ligands ensure strong binding with most impurities over a wide range of pH and salt concentrations.The core of each bead is functionalized with ligands that are both hydrophobic and positively charged, resulting in a highly efficient multimodal binding of various contaminants small enough to enter the core.These larger molecules are collected in the column flowthrough while smaller impurities bind to the internalized ligands.The inactive shell excludes large molecules (cut off ∼ Mr 700000 ) from entering the core through the pores of the shell.Capto Core 700 is composed of a ligand-activated core and inactive shell.Convenient small-scale purification, process development, and scale-up using prepacked HiTrap and HiScreen columns.Straightforward optimization due to flowthrough chromatography and robust performance. Structure and functional properties of Capto Core 700 core-shell particles J Chromatogr A.Significantly improved productivity and higher flow rates compared with gel filtration methods.Novel core bead technology allows efficient capture of contaminants while target molecules are collected in the flowthrough. The 3 steps purification protocol uses a recently developed multimodal size-exclusion chromatography medium (Capto Core 700) in combination with detergent extraction and size-exclusion polishing to reach a 89 VLP purity with a 19 yield.For intermediate purification and polishing of viruses and other large biomolecules (Mr > 700 000) in flowthrough mode.Use water spray to keep fire-exposed containers cool. Move containers from fire area if this can be done without risk. No action shall be taken involving any personal risk or without suitable training. The core of each bead is functionalized with hydrophobic and positively charged ligands. The inactive shell aids in preventing large molecules (cut off Mr 700 000) from entering the core via the pores of the shell. The novel core bead technology and multimodal, octylamine ligand give Capto Core 700 dual functionality, size exclusion, and binding chromatography in one chromatography medium (resin). Capto Core 700, 100 ml 17548102 Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. Capto Core 700 comprises a ligand-activated core and inactive shell.
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